4. You are preparing 250 mL of gel mix for protein electrophoresis, which requires a final concentration of 1.6% SDS. The lab has a 10% stock solution of SDS. How much SDS stock solution (in mL) do you use? Round your answer to the nearest whole number. (Ch. 2.1)

Question

Chemistry

Ronald Rush

24 October, 02:30

4. You are preparing 250 mL of gel mix for protein electrophoresis, which requires a final concentration of 1.6% SDS. The lab has a 10% stock solution of SDS. How much SDS stock solution (in mL) do you use? Round your answer to the nearest whole number. (Ch. 2.1)

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Answers (1)

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Ali Levy · Answer 1

40 mL

Explanation:

To prepare a solution by a concentrated solution, we must use the equation:

C1xV1 = C2xV2

Where C is the concentration, V the volume, 1 is the initial solution and 2 the final solution. So, the final volume will be 250 mL, with 1.6% of concentration:

10xV1 = 1.6x250

V1 = 40 mL

So, it's necessary 40 mL of the SDS stock solution.