When NEM is added to a purified solution of creatine kinase, Cys 278 is alkylated, but no other Cys residues in the protein are modified. What can you infer about the Cys 278 residue based on this observation

Question

Chemistry

Janiyah Alvarado

2 May, 19:53

When NEM is added to a purified solution of creatine kinase, Cys 278 is alkylated, but no other Cys residues in the protein are modified. What can you infer about the Cys 278 residue based on this observation

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Answers (1)

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Blaze Bush · Answer 1

The answer is given below

Explanation:

Cys 278 residue is the only available cysteine which is alkylated by the addition of N-Ethylmaleimide or NEM (alkylating agent). It works by only alkylating the sulfhydryls. In this case, Cys 278 residue is the only one which has exposed cysteine residue.

While the other residues have their sulfhydryls group either involved in the synthesis of disulfide bonds of proteins or their Cys residues are intrinsically placed in the proteins and cannot be alkylated with NEM.

NEM cannot alkylate if its protein is not available in the free form or it is in bounded form. For NEM to alkylate Cys 278, it should be free and should have sulfhydryls available for alkylation.

Alkylation: it is the transfer of alkyl groups. Alkyl groups contain Hydrogen and Carbon in their structure.