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21 July, 11:50

A calibration curve relating absorbance (y) to concentration (x, measured in mol/L) of y = 0.36x-0.011 Use the calibration curve to determination of the concentration of a solution with an absorbance of 0.02.

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  1. 21 July, 12:17
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    The concentration is 0.025 M.

    Explanation:

    We will use beer law to find the concentration.

    A = εmCl

    This law relate to the linear equation Y = mx + b in following way,

    A = y = absorbance

    m = εm = slope or extinction coefficient in beer law.

    x = c = concentration

    and l is length which is 1 cm

    Thus equation can be written as,

    A = εmc + b

    A - b / εm = c

    in order to find the concentration we will abstract the intercept from absorbance and then divide it by the slop.

    0.02 - 0.011 / 0.36 = c

    c = 0.009

    c = 0.025 M.
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