A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation?=0.0182x+0.007 where? is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms (μg). Determine the quantity of protein in a solution that has an absorbance of 0.276. A blank solution has an absorbance of 0.061.

Question

Chemistry

Ross Pugh

9 May, 15:50

A calibration curve was created to determine the quantity of protein in a solution. The calibration curve has the form of a straight line with the equation?=0.0182x+0.007 where? is the corrected absorbance of the solution and x is quantity of protein in the solution in units of micrograms (μg). Determine the quantity of protein in a solution that has an absorbance of 0.276. A blank solution has an absorbance of 0.061.

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Answers (1)

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Gael Gallagher · Answer 1

The solution has 11.4 μg protein

Explanation:

Hi there!

The corrected absorbance will be the measured absorbance minus the absorbance of the blank that is a solution without protein.

Then, the corrected absorbance will be:

0.276 - 0.061 = 0.215

Now, using the equation of the calibration curve, we can obtain the quantity of protein present in the solution:

0.215 = 0.0182x + 0.007

(0.215 - 0.007) / 0.0182 = x

x = 11.4 μg

The solution has 11.4 μg protein