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5 June, 14:29

Assume the molar absorptivity (ε) for CoCl42 - at 700 nm is 409.7 M-1 cm-1. If d, the cell path length, is equal to 1.48 cm for the cuvettes being used, and if the measured absorbance (A) at 700 nm is 1.147, what is [CoCl42-]?

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  1. 5 June, 18:01
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    1.89 * 10⁻³ M

    Explanation:

    In a UV Visible Spectrometer, we can determine the concentration of a species using Beer-Lambert law.

    A = ε * l * c

    where,

    A: absorbance ε: molar absorptivity l: the cell path length c: concentration of the species

    We can find [CoCl₄²⁻] from this expression.

    A = ε * l * c

    c = A / ε * l

    c = 1.147 / 409.7 M⁻¹ cm⁻¹ * 1.48 cm

    c = 1.89 * 10⁻³ M
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