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13 August, 17:44

During the GFP purification experiment, the instructor will have to make breaking buffer for the students to use. This buffer contains 150mM NaCl. Given a bottle of crystalline NaCl (M. W. = 40g/mol), describe how you would make 500ml of 150mM NaCl. Include the type and sizes of any measuring vessels that you use to make the solution.

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  1. 13 August, 20:08
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    150 mM NaCl = 0.15 M NaCl

    500 ml of 0.15 M NaCl = 500 x 0.15 ml of M NaCl

    = 75 ml of M NaCl

    M. W = 40 g

    1000 ml of M NaCl requires 40 g

    75 ml of M NaCl requires (40/1000) x75

    = 3 g

    We shall take 500 ml of water in a graduated cylinder. Then we weigh 3 g of salt and add it to 500 ml of water and mix it. We shall get 500 ml of 150mM NaCl.
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