Identify the applications for gel filtration chromatography. separation of components in a mixture by size estimation of molecular weights separation of components in a mixture by hydrophobicity purification of a specific protein from a mixture

The question is complete but its options are no properly separated

Question:

Identify the applications for gel filtration chromatography.

1. Separation of components in a mixture by size

2. Estimation of molecular weights

3. Separation of components in a mixture by hydrophobicity

4. Purification of a specific protein from a mixture

Answer:

1. Separation of components in a mixture by size

2. Estimation of molecular weights

Explanation:

Gel filtration chromatography (GFC) is a type of size exclusion chromatography in which the molecules are separated based on their size, and the mobile phase is water. It consists of a stationary phase that has molecular sieves. These molecular sieves are contained in a column and are usually made of polymers networks. The size of the sieves in the column is determined by the degree of crosslinking in the stationary phase polymers.

This technique can be used for the estimation of the molecular weight of the polymers. The separation of molecules is based on the size and the shape of the molecule, which correlates with the molecular weight.

With the above information let's review the options

Options 1 and 2, agree with the above description of gel filtration chromatography.

Options 3 and 4 are not the applications of GFC, as they are not separated based on their size.