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30 December, 15:53

Calculate the volume of compound X needed to ma ke a 15 ug/ml treatment solution in 0.1 L of 1x PBS using a stock solution that contains 10 ml of compound X at a concentration of 250 mg/ml. 60 ul 6 ml

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  1. 30 December, 19:28
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    6 μL

    Explanation:

    To prepare a solution by a concentrated solution, we must use the equation:

    C1xV1 = C2xV2, where C is the concentration, V the volume, 1 is the initial solution and 2 the final solution.

    The final concentration must be 15 μg/mL with a volume of 0.1L or 100 mL. The concentration of the stock solution is 250 mg/mL. 1 mg has 1000 μg, so the concentration is 250000 μg/mL. Then:

    250000xV1 = 15x100

    V1 = 15/2500

    V1 = 0.006 mL = 6 μL
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