A specific steroid has a λmax of 282 nm and a molar absorptivity ε of 11, 500 L mol - 1 cm - 1. What is the concentration of the compound in a solution whose absorbance at 282 nm is A = 0.060 with a sample pathlength of 1.00 cm?

Question

Chemistry

Madeline Wilkins

20 September, 08:28

A specific steroid has a λmax of 282 nm and a molar absorptivity ε of 11, 500 L mol - 1 cm - 1. What is the concentration of the compound in a solution whose absorbance at 282 nm is A = 0.060 with a sample pathlength of 1.00 cm?

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Answers (1)

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Phoenix Greene · Answer 1

The molar concentration of the compound is 5.2 x 10⁻⁶ M

Explanation:

According to Beer-Lambert law the concentration of the sample and the sample path length have a linear relationship with the light absorbed at a particular wavelength.

This problem can be solved by this equation, which is as follows,

A = ε. b. C

Here, A is the absorbance, ε is the molar absorptivity, b is the sample path length and C is the molar concentration

0.060 = 11500 x (1) x C

C = 5.2 x 10⁻⁶ M