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30 August, 21:13

In a paper chromatography analysis, three pigments, A, B, and C, were dissolved in a solvent. A is slightly polar, B is highly polar, and C is moderately polar. List in order how these will appear on the surface of the chromatography strip starting from the applied spot at the origin line.

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  1. 30 August, 21:52
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    Answer:It would depend on the eluent solvent what we use, If we would use a polar solvent than B will be appear first followed by c and then A on the chromatography strip.

    If we use non-polar eluent solvent than A will be separated first followed by C and thenB on the chromatography strip.

    Explanation:

    Separation using paper chromatography is dependent upon the polarity of various pigments.

    A polar pigment would move ahead in case of polar solvent used and a non-polar pigment would move ahead when we use a non-polar solvent.

    So separation would occur in order of polarity of various pigments in a given solvent.

    The amount of distance travelled by each component (or pigment or spot) can be calculated using the formula for retention factor:

    Rf = Distance travelled by pigment spot or solute/Distance travelled by eluent solvent

    Rf = Retention factor

    Retention factor is basically the ratio of distance tarvelled by the pigment or spot to the ratio of distance travelled by the solvent.
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