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2 November, 15:06

The first solution will be an unbuffered sucrose solution. Calculate the amount of 0.1 M sucrose stock solution and the amount of dH2O needed to prepare 100 mL of unbuffered 20 mM sucrose.

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  1. 2 November, 16:01
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    20mL of the stock solution and dilute to 100mL

    Explanation:

    20mM = 0.020M is the concentration of the diluted solution. As you want to prepare this solution from a 0.1M solution, dilution factor is:

    0.1M / 0.020M = 5

    That means you need to dilute the stock solution 5 times to obtain the 20mM sucrose solution.

    As you want to prepare 100mL, you need to add:

    100mL / 5 =

    20mL of the stock solution and dilute to 100mL
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