What region in North America became a cause of conflict between France and Great Britain before the French and Indian War?

One region in North America that became the reason of conflict between France and Great Britain was Ohio Valley region.

Explanation:

The French and Indian war was a war that fought between the British America and New France. The war started in 1754 and ended in 1754. In this war both sides were supported by the militaries of their sides along with the Native American allies. In this was British American side was more powerful as they had 2 million colonies as compared to French colonies.

Ohio River Valley