What is the temperature used for the extension step?

72°C

Explanation:

The polymerase chain response (PCR) could be a strategy to quickly open up groupings of DNA. Amid a normal PCR, format DNA (containing the locale of intrigued) is blended with deoxynucleotides (dNTPs), a DNA polymerase and small segments of DNA called primers. PCR involves a series of temperature cycles. The temperature used for the extension step is 72°C. The temperature that is used during the extension phase is dependent on the DNA polymerase that is used. Amid the expansion step (ordinarily 68°C-72°C) the polymerase amplifies the groundwork to create an early DNA strand. This prepare is rehashed different times Generally at this stage, the reaction mixture is heated to a temperature intermediate between the denaturation and annealing temperatures. The optimal temperature for Taq polymerase is 72°C.