The tac promoter, an artificial promoter made from portions of the trp and lacUV5 promoters, has been introduced into a plasmid. It is a hybrid of the lac and trp (tryptophan) promoters, containing the - 35 region of one and the - 10 region of the other. This promoter directs transcription initiation more efficiently than either the trp or lac promoters. Why

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Physics

Hanna Key

20 May, 10:19

The tac promoter, an artificial promoter made from portions of the trp and lacUV5 promoters, has been introduced into a plasmid. It is a hybrid of the lac and trp (tryptophan) promoters, containing the - 35 region of one and the - 10 region of the other. This promoter directs transcription initiation more efficiently than either the trp or lac promoters. Why

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Kenny Morris · Answer 1

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The tac promoter is more efficient than the trp and lac UV5 because as opposed to the trp and lac UV5 promoters, the tac promoter has both a consensus - 35 sequence (a nucleotide sequence of DNA, RNA or amino acids used for inter or intra molecular interactions) and a consensus pribnow box sequence (a prokaryotic sequence with core element at - 10 position).