Why do the pvc plastic wells used in this weeks elisa need to be sticky on the inner walls and how may your results change if a different non-adherent plastic was used instead?

Question

Physics

Luz Reeves

2 April, 15:52

Why do the pvc plastic wells used in this weeks elisa need to be sticky on the inner walls and how may your results change if a different non-adherent plastic was used instead?

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Kiara Lawrence · Answer 1

The stickiness in the inner walls allows them to be easily coated with the desired antigens, this translates in the use of a smaller amount of antigen. If the walls weren't sticky there's a possibility the antigen won't stick to them and therefore the result of the ELISA can be a false negative.

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