Which of the following is not a true statement comparing prokaryotic and eukaryotic DNA replication?

DNA replication always occurs in the nucleus.

Eukaryotic DNA replication involves more polymerases than prokaryotic replication.

Eukaryotic DNA replication requires multiple replication forks, while prokaryotic replication uses a single origin to rapidly replicate the entire genome.

Both eukaryotic and prokaryotic DNA polymerases build off RNA primers made by primase.

DNA replication always occurs in the nucleus.

Explanation:

Prokaryotes lack the membrane-bound organelles. The nucleus is a double membrane-bound organelle and is absent in prokaryotic cells. The DNA of prokaryotes is present in the cytoplasm itself. Therefore, DNA replication in prokaryotes does not occur in the nucleus.

On the other hand, the DNA of eukaryotes is present in a well-defined nucleus. DNA replication in eukaryotes occurs in the nucleus; not in the cytoplasm. For example, DNA replication in humans occurs in the nucleus but the process takes place in the cytoplasm of a bacterial cell.