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3 June, 22:45

A 1.5-kb fragment of DNA is cloned into the EcoRI site of a plasmid vector that is 5.5-kb long. The plasmid vector is then transformed into bacteria. If the plasmid DNA is then extracted from a single bacterial colony and digested with EcoRI, what digestion products will be produced if the plasmid lacks the fragment?

A) one 5.5-kb fragment

B) one 1.5-kb fragment

C) one 7-kb fragment

D) one 1.5-kb fragment

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  1. 4 June, 01:50
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    A) ? I would assume this because the plasmid lacks the fragment.
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