A scientist has sequenced a gene from a multicellular eukaryotic organism. Brendan claims that by analyzing the gene sequence and applying

the genetic code, he can conclude the sequence of amino acids in the protein that forms after the gene is transcribed and translated

Which statement provides the strongest evidence against the validity of Brendan's claim?

O A Regulatory proteins may activate or deactivate a gene, often by very complex mechanisms.

B. During transcription, a strand of mRNA is synthesized from complementary base pairs, with the substitution of uracil (U) for thymine

(1)

OC. During translation, anticodons on tRNA bind to codons on mRNA as part of the process that forms the polypeptide

o

D. After a gene is transcribed, introns are removed from the pre-mRNA to form the mRNA that is translated.

D. After a gene is transcribed, introns are removed from the pre-mRNA to form the mRNA that is translated.

Explanation:

The statement that provides the strongest evidence against the validity of Brendan's claim is that after a gene is transcribed, introns are removed from the pre-mRNA to form a mRNA that is then translated.

A newly transcribed gene usually have two group of nucleotides - the exons and the introns. Exons are otherwise known as he coding sequence of a gene while introns are known as the non-coding sequence of a gene.

After transcription, the introns are excised out of the nucleotide sequence of the newly produced mRNA. The remaining nucleotides are then spliced before they are transported to the cytoplasm to be translated into proteins.

Hence, the sequence of amino acids in a protein that will be formed by a pre-mRNA cannot be determined until the non-coding sequence are removed.

The correct option is D.