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24 August, 09:47

What is the function of a mordant in gram staining?

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  1. 24 August, 10:03
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    The function of mordant in the gram staining is to expose grams positive cell to the decolorizer which dissolves the lipids in the cell wall thus allowing the crystals violent-iodine to leach out of the cell. This facilitate the cell subsequently be stained by with safranin.
  2. 24 August, 13:27
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    The function that a mordant has is to form a complex with the primary stain so that it becomes trapped with the bacteria that have structural properties. In Gram stain, the gram of iodine is used to form a complex with crystal violet, which becomes trapped in gram-positive cells. For this reason, its color is purple.
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