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9 December, 16:10

If a 0.10 M solution of a colored substance has a maximum absorbance at 500 nm and an absorbance of 0.26 M at this wavelength, what will be the measured absorbance of a 0.20 M solution at 500 nm?

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  1. 9 December, 17:13
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    Absorbance is related to the concentration of a substance using the Beer-Lambert's Law. According to this law, absorbance is linearly related to concentration. However, this is only true up to a certain concentration depending on the substance. For this case, we assume that the said law is applicable.

    A = kC

    Using the first conditions, ewe solve for k.

    0.26 = k (0.10)

    k = 2.6

    A = kC

    A = 2.6 (0.20) = 0.52

    Therefore, the absorbance at a concentration of 0.20 M and wavelength of 500nm is 0.52.
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