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22 April, 13:18

If you suspect your culture of bacteria has 25 x 107 cells per mL, what would you want the final dilution to be in order to end up with 100 cfu on a plate?

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  1. 22 April, 13:36
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    dilution = 2.5*10^6

    Explanation:

    Given:

    - Number of colonies N = 100 cfu

    - The Volume of cells = 25*10^7 cells / ml

    Find:

    the final dilution

    Solution:

    - The expression relating the the colonies forming unit with cells//volume is given as:

    Bacteria/ml = cfu*dilution

    dilution = Volumetric cells / cfu

    dilution = 25*10^7 / 100

    dilution = 2.5*10^6
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